Energy-resolved HCD fragmentation of daunorubicin-peptide conjugates

my field is peptide bioconjugates chemistry. I have an article in bioconjugates analytical chemistry
I submitted my article before one month to the journal mass spectrometry, but the Editor board want a major revision ) and ask me many questions about my paper .it means the reviewers in this journal asked a lot of different questions and changes and some comments which are I must be to answer satisfactorily.

I would like to ask you to answer satisfactorily for all comments my the manuscript and questions in order to accept my article as soon as possible in this journal.
I attached 2 docs the first one the original manuscript
and the second file involve questions and comments I received from editor board the journal

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