Introduction/background lab paper (2 and 1/2 pages) about the following; explaining what are these techniques? What each is used for; functions? Why is it important? Use the references in the attachment and first of all check the instruction file !!
1. ORF fusions between GFP and a Target Gene
2. pPEM109
3. LEPA/EF4; elongation factor 4 (EF-4) refer to the gene as lepA
4. Tn5 Vitro transposition reaction: http://www.jbc.org/content/273/13/7367.full
5. TRANSFORMATION OF TRANSPOSITION MIXTURE INTO E. coli.
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