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The Effect of low pH on Enzyme Activity
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project it may be useful for you to first review the Scientific Method Tutorial informationabout pH and enzymes in the text book and course modules Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4 you will be reminded that there are several factors that impact enzymatic activity: pH temperature and amount of reagent.It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment) or you can search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references).
As you design your experiment for this project please remember that you are trying to examine how anacidic fluidwill modify the outcome of an enzymatic reaction. To successfully complete this project you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design you must clearly explain what you are doing. That means that youwill need to identify the enzyme the substrate the acidic fluid used as treatment the control treatment and the method of measuring enzyme activity as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the text book modules lab manual and potentially additional information sources.
Hint:Keep in mind that the acid will change the environmental conditions of the experiment (for example a low pH value could change the shape of the active site on the enzyme protein) without directly participating in the reaction.
Lab Materials
Youmayneed all or some of the following depending on your experimental design:
Materials from your lab kit:
You may choose to use additional materials (different acidic fluids and/or different organisms and/or different substrate if you chose an enzyme other than catalase).
Outline:
Include the following in your outline:
Write a lab report that includes the following:
1. Title page: descriptive title your name course name semester
2.Introduction: general background information about enzymes and specific information about your chosen enzyme the question(s) that you are asking and a clear hypothesis for your experiment (20 points).
3. Design an experiment. Provide a detailed description of the materials and methods used to conduct the experiment. Identify control and experimental samples as well as independent and dependent variables. Also include the methods used for data collection and analysis (20 points).
4. Conduct the experiment and record your results. Take picture(s) of your results. What did you observe?Present your data in table and/or graph format. Remember to label everything and include the unit of measure with all numbers (20 points)
5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary for you to refer to the OLI course modules lab manual and/or use additional information resources. What effect does the acidic treatmenthave on enzyme activity? Did you get the expected results? Explain. (20 points)
6.State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back how could you have improved your experiment?(10 points)
7. Include a list of references to all information sources used in APA format (5 points).
Avoid Plagiarism
It is very important to write withyour own words. If you do copy one or two sentences directly (use sparingly) use quotation marks () around the copied text followed by an in-text reference. All information sources need to be included in the reference list and as in-text references. Plagiarism will be reported to the proper UMUC authority. Guidance on how to avoid plagiarism can be found here:https://www.umuc.edu/students/academic-integrity/ai-tutorial/academic-integrity-tutorial.html
Submission
Submit your final applied lab project in the assignment folder by the due date specified in the course schedule.
Grading
Your Outline will be graded based on the following criteria (100 points total):
Excellent (90-100%)
Good (70-89%)
Adequate (60-69%)
Unsatisfactory (0-59%)
Outline
100 points
Outline includes all the required components and planned experiment meets requirements for the assignment and is clearly and accurately described. Submitted on time.
90-100 points
Outline missing one or two of the required componentsand/orplanned experiment does not meet one of requirements for the assignmentand/orminor issues with clarity and accuracy.
70-89 points
Outline missing several of the required componentsand/orplanned experiment does not meet several of the requirements for the assignmentand/ormajor issues with clarity and accuracy.
60-69 points
Outline missing most of the required componentsandplanned experiment does not meet the requirements of the assignment.
0-59 points
Your Final Applied Lab Project will be graded based on the following criteria (100 points total):
Excellent (90-100%)
Good (75-89%)
Adequate (50-74%)
Unsatisfactory(0-49%)
Introduction Question and Hypothesis
20 points
Background information about enzymes in general and about specific enzyme used in project is clearly and accurately written. Questions and hypothesis are specific relevant and clearly stated.
18-20 points
Background information about enzymes in general and about specific enzyme is somewhat unclear and/or inaccurate. Questions and hypothesis could be more specific relevant and clearly stated.
15-17 points
Missing background information about enzymes in generalorabout specific enzyme used in projectorquestions and hypothesis.
10-14 point
Missing background information about enzymes in generalandabout specific enzyme used in projectandquestions/hypothesis.
0-9 points
Experimental Design Materials and Methods
20 points
Experiment is designed to directly test the hypothesis; description of experiment is detailed and well written and includes all materials and methods used.
18-20 points
Experiment is mostly designed to test the hypothesisand/ordescription of experiment is somewhat inaccurateand/orsome information about materials and methods used is missingand/orminor problems with clarity an organization.
15-17 points
Experiment is barely designed to test the hypothesisand/ordescription of experiment is inaccurateand/ormost of the materials and methods used are missingand/ormajor problems with clarity an organization.
10-14 points
Experiment is not designed to test hypothesisand/ordescription of experiment and materials and methods used are missing.
0-9 points
Results
20 points
Results are clearly and accurately presented in a table and/or graph and picture(s).
18-20 points
Results are presented but minor problems with clarity and/or accuracy.
15-17 points
Results are described but major problems with clarity and/or accuracy and/or resultsnot presented intable or graph.
10-14 points
Results are not included
0-9 points
Discussion of Results
20 points
A clear accurate and well organized discussion of results that demonstrates good knowledge of enzymes.
18-20 points
Minor problems with clarity accuracy or organizationand/ordemonstrates some gaps in knowledge of enzymes.
15-17 points
Major problems with clarity accuracy or organizationordemonstrates minimal knowledge of enzymes.
10-14 points
Missing discussion of results or major problems with clarity accuracy or organizationandmissing understanding of enzymes.
0-9 points
Conclusion
10 points
A well stated conclusion that is accurate and specific and directly related to the data collected. Clearly stated whether or not the results support the hypothesis. Relevant suggestion for improvement of experiment.
9-10 points
Conclusion stated but slightly inaccurateand/ornot directly related to data collectedand/orsuggestion for improvement of experiment not very relevant.
7-8 points
Conclusion stated but inaccurateand/orbarely related to data collectedand/ormissing suggestion for improvement of experiment.
5-6 points
Missing conclusion only suggestion for improvement of experiment.
0-4 points
Citations
5 points
All references used are included and in correct APA format in-text references are included.
5 points
All references are included but a few minor mistakes in APA formatand/ormissing in-text references.
4 points
Missing one or several referencesand/ormajor mistakes in APA format.
2-3 point
No references included or references not in APA format.
0-1 points
Grammar and Spelling
5 points
0 – 3minorspelling and grammatical errors
5 points
Between 4 and 7 minor spelling and grammatical errors
4 points
Between 8 and 12 spelling and grammatical errors
3 point
More than 13 spelling and grammatical errors
0-2 points
Hide Rubrics
Rubric Name: Final Applied Lab Project
Background information about enzymes in general and about specific enzyme used in project is clearly and accurately written. Questions and hypothesis are specific relevant and clearly stated.
18-20 points
Background information about enzymes in general and about specific enzyme is somewhat unclear and/or inaccurate. Questions and hypothesis could be more specific relevant and clearly stated.
15-17 points
Missing background information about enzymes in generalorabout specific enzyme used in projectorquestions and hypothesis.
10-14 point
Missing background information about enzymes in generalandabout specific enzyme used in projectandquestions/hypothesis.
0-9 points
Experiment is designed to directly test the hypothesis; description of experiment is detailed and well written and includes all materials and methods used.
18-20 points
Experiment is mostly designed to test the hypothesisand/ordescription of experiment is somewhat inaccurateand/orsome information about materials and methods used is missingand/orminor problems with clarity an organization.
15-17 points
Experiment is barely designed to test the hypothesisand/ordescription of experiment is inaccurateand/ormost of the materials and methods used are missingand/ormajor problems with clarity an organization.
10-14 points
Experiment is not designed to test hypothesisand/ordescription of experiment and materials and methods used are missing.
0-9 points
Results are clearly and accurately presented in a table and/or graph format.
18-20 points
Results are presented but minor problems with clarity and/or accuracy.
15-17 points
Results are described but major problems with clarity and/or accuracy and/or resultsnot presented intable or graph.
10-14 points
Results are not included or major problems with clarity accuracy or organization
0-9 points
A clear accurate and well organized discussion of results that demonstrates good knowledge of enzymes.
18-20 points
Minor problems with clarity accuracy or organizationand/ordemonstrates some gaps in knowledge of enzymes.
15-17 points
Major problems with clarity accuracy or organizationordemonstrates minimal knowledge of enzymes.
10-14 points
Missing discussion of results or major problems with clarity accuracy or organizationandmissing understanding of enzymes.
0-9 points
A well stated conclusion that is accurate and specific and directly related to the data collected. Clearly stated whether or not the results support the hypothesis. Relevant suggestion for improvement of experiment.
9-10 points
Conclusion stated but slightly inaccurateand/ornot directly related to data collectedand/orsuggestion for improvement of experiment not very relevant.
7-8 points
Conclusion stated but inaccurateand/orbarely related to data collectedand/ormissing suggestion for improvement of experiment.
5-6 points
Missing conclusion only suggestion for improvement of experiment.
0-4 points
All references used are included and in correct APA format in-text references are included.
5 points
All references are included but a few minor mistakes in APA formatand/ormissing in-text references.
4 points
Missing one or several referencesand/ormajor mistakes in APA format.
2-3 point
No references included or references not in APA format.
0-1 points
0-3minorspelling and grammatical errors
5 points
Between 4 and 7 minor spelling and grammatical errors
4 points
Between 8 and 12 spelling and grammatical errors
3 point
More than 13 spelling and grammatical errors
0-2 points
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