Outline the principle of Electrospray Ionisation.Dear Sir/ Madam,First of all, I want you to write the introduction, the introduction includes: 1) Theory. 2) Instrumentation. 3) Applications.
Dear Sir/ Madam,First of all, I want you to write the introduction, the introduction includes: 1) Theory. 2) Instrumentation. 3) Applications.The second thing is: the aim of an experiment.The third thing: I will write this, the procedure, ( as manual, but some changes mad which are Bileacid sample rather than Phenylalanine and Tyrosine, mobile phase A and B was ( A: methanol 7.5mM amonimacetat) also 75% mobile phase A and 25% mobile phase B), also no need to fragmentor voltage, Ion source Dual ESI ( Negative moode), the wavelength will not be in results, and injection volume is 5μl.Fourth thing: the results, it are in the attachment.Fifth thing: discussion.What I want to discuss are in the last page of manual, but there are some changes. So you will discuss what I write it down:1) Outline the principle of Electrospray Ionisation. In what situations would you use an alternative ion source (e.g. Atmospheric Pressure Chemical Ionisation)? Why is a vacuum required in mass spectrometry?2) What effect does working in positive and negative ion mode have on the m/z values? Why was negative ion mode selected?3) What effect does changing the collision energy have on the MS sensitivity? What effect does too high a collision energy have on the ions?4) What are the main advantages of LC-ESI-MS as a tool for identifying compounds?and also mention that from the results :what type separation?. Our sample contain 8 different ions from bileacid, I need to compare that each ion with anther one ( for example why the first come at the end, why the third one come before the second one) like this.I need to judge on these in general. The last thing is label the peaks on the first picture in the results.Also, I try to ask the demonstrator son questions and he give me this note, “Note: the first chromatogram on page 1 is the TIC (total ion current) chromatogram and all of the others are EIC (extracted ion current) chromatograms for specific m/z species. Importantly, the last EIC chrom. on the 3rd page is the only one you need (on that page), as the first three EIC chrom.s on the 3rd page are the same as the last three on the 2nd page.Please include the possible reason(s) for the order of elution observed for the different bile acids.”so you will work on the introduction, write the aim and the discussion with label the peaks. Pleas mention all what the demonstrators want .Not: The report must be without plagiarism and with references. And the manual for the experiment in the attachment with the results.If you have any question please send to me.
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